Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-osteoporosis effect of Notopterygium incisum Ting ex H. T. Chang extract in rats

Hai Ding , Jian Yao, Wenju Chang, Fendou Liu, Qingliang Zeng, Zhenxing He

Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, PR China;

For correspondence:- Hai Ding Email: dinghai133@126.com Tel:+865523086163

Accepted: 16 September 2019 Published: 31 October 2019

Citation: Ding H, Yao J, Chang W, Liu F, Zeng Q, He Z. Anti-osteoporosis effect of Notopterygium incisum Ting ex H. T. Chang extract in rats. Trop J Pharm Res 2019; 18(10):2051-2055 doi: 10.4314/tjpr.v18i10.8

© 2019 The authors.
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Abstract

Purpose: To investigate the effect of Notopterygium incisum Ting ex H.T. Chang extract (NICE) on ovariectomy-induced osteoporosis in rats.

Methods: Female Sprague-Dawley rats were randomly assigned to a normal group (control) and five ovariectomy (OVX) subgroups: OVX with vehicle (OVX), OVX with positive control drug alendronate sodium tablets (1.8 mg/kg/week), and OVX with varying NICE doses (45, 90, or 180 mg/kg/day). After rats were subjected to ovariectomy for 4 weeks, alendronate or NICE were administered orally daily for 16 weeks. The bone mineral density (BMD) of the L4 vertebrae and right femurs of all rats was estimated. The serum hormones estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels and serum alkaline phosphatase (ALP), osteocalcin (OC) and telopeptides of collagen type I (CTx) levels of rats were detrmined.

Results: The results show that NICE dose-dependently inhibited bone mineral density (BMD) reduction of L4 vertebrae and femurs (p < 0.05). Compared with OVX group, serum E2, FSH and LH levels was significantly increased in osteoporosis rats (p < 0.01), but serum ALP, CTx, and OC concentrations were significantly lower (p < 0.05). On the other hand, bone trabeculae in the three NICE groups and nilestriol group were all wider, while the space and connections increased.

Conclusion: These findings indicate that NICE mitigates OVX-induced osteoporosis in rats, and hence can potentially be developed for the management of osteoporosis.

Keywords: Notopterygium incisum, Osteoporosis, Ovariectomy, Bone tissue morphology